5 Simple Statements About thr77 Explained
5 Simple Statements About thr77 Explained
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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene continues to be observed in several tumors, which ends up in chromosome instability, and thus might add to tumorigenesis. This protein was observed to associate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a significant position in advertising and marketing cell-cycle progression from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are described. Two supplemental splice variants ended up noted but in-depth nucleotide sequence data isn't still offered.
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Blocking peptides are peptides that bind precisely towards the focus on antibody and block antibody binding. These peptide usually is made up of the epitope regarded from the antibody.
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This guideline outlines further more feasible optimization of mobile and immunoassay parameters to guarantee the absolute best final results are...
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This guidebook outlines even more doable optimization of mobile and immunoassay parameters to guarantee the very best success are...
Antibodies sure to the blocking peptide now not bind into the epitope over the concentrate on protein. By evaluating the staining with the blocked antibody versus the antibody on your own, one can see which staining is certain.
Target Information and facts Cyclin E belongs into the extremely conserved cyclin relatives, whose users are characterized by a remarkable periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Various cyclins show distinctive expression and degradation styles which contribute into the temporal coordination of every mitotic party. Cyclin E types a posh with and functions like a regulatory subunit of CDK2, whose exercise is required for mobile cycle G1/S changeover. This protein accumulates in the G1-S stage boundary and is particularly degraded as cells progress by way of S section.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by the two antibodies...
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